Equipment

The Nikon Yokogawa CSU-W1 spinning disk confocal microscope is an automated live-cell imaging system designed for fast high-resolution confocal 2D or 3D aquisition of images and time series. Its dual-disk CSU-W1 scan head, sensitive dual sCMOS camera detection, environmental control, Perfect Focus System, piezo Z-drive, and long-working-distance water immersion objectives enable deep imaging of delicate 3D biological samples with low phototoxicity. The system supports adaptive feedback microscopy through integrated acquisition and analysis software, allowing real-time image analysis to guide automated imaging of dynamic processes and rare events.

• Long-term live-cell imaging with environmental control
• Fast 3D confocal imaging of delicate biological samples with reduced phototoxicity
• Adaptive feedback microscopy for rare events
• Automated multi-position and multiwell plate imaging
• High-resolution 3D imaging of individual cells and subcellular structures
• Imaging-based screening and analysis
• Simultaneous 2-color imaging (dual cam configuration)

• High-end system based on the Nikon Ti2-E inverted motorized research microscope platform
• Yokogawa CSU-W1 confocal spinning disk scan head, dual-disk (25 µm and 50 µm pinholes) in dual-camera configuration with variable speed.
• Two Hamamatsu ORCA-Fusion BT back-illuminated sCMOS cameras (5.3 megapixel monochrome sensor with 2304 x 2304 pixels, pixel size 6.5 µm, quantum efficiency > 95%, 23.2 frames per second full frame)
• Autofocus (Nikon Perfect Focus System 4)
• Motorized x-y stage with linear encoders(step size 100 nm, repeatability ± 500 nm, travel speed 25 mm/s)
• Supports fluorescence (confocal, widefield) and transmitted light modes (brightfield, DIC)
• Environmental control for temperature, CO₂ and humidity.
• Automated water immersion dispenser
• Adaptive workflow automation NIS JOBS module for programmable workflows, multiwell scanning, interactive measurements, real-time analysis, and conditional acquisition
• Analysis software NIS General Analysis 3
• AI analysis software NIS.ai module with Enhance.ai, Convert.ai, and Segment.ai

Specifications for Lasers, Filters, and Lenses

The Sony ID7000 is a high-parameter spectral flow cytometry analyzer for rapid, sensitive fluorescence measurements in individual cells. In the configured 4-laser setup, it records full spectral fluorescence profiles and uses spectral unmixing to separate overlapping fluorophores and remove autofluorescence, enabling detection of weak fluorescent signals. The system combines high-throughput automated sample acquisition from tubes and multi-well plates.

• High-throughput single-cell fluorescence analysis of fixed and live cells
• Detection of weak endogenous fluorescent protein signals in cells with high autofluorescence
• Spectral unmixing of overlapping fluorophores
• Automated analysis of large sample series in tubes, 96-well plates, and 384-well plates
• Broad compatibilty and optimal detection of diverse fluorophores 

• Available laser wavelengths: 405 nm, 488 nm, 561 nm, 637 nm
• Total of 112 photomultiplier tube detectors for spectral analysis
• Aquisition of fluorescence spectra, forward scatter and side scatter
• Event rate up to 35,000-40,000 events per second
• Sample compatibility with fixed and live cells
• Auto Sampler compatible with 5 ml tubes (12 x 75 mm) as well as 96-well and 384-well plates
• Integrated thermoelectric Peltier cooling and active agitation for all sample formats
• Real-time spectral unmixing and data visualization
• Fluorescence sensitivity: FITC ≤ 58 MESF; PE ≤ 4 MESF; APC ≤ 7 MESF

Full specifications