HPLC/Mass Spectrometry
Lab management: Stephanie Schulz
The HPLC/MS department is part of the organic chemistry, but samples are accepted and processed by the entire department.
The equipment includes one preparative, two analytical and two semi-preparative HPLC systems from Knauer. Furthermore, a Waters HPLC system with coupled mass spectrometer and TLC-MS interface is available.
Analytical HPLC systems
The first system consists of:
- a Smartline Pump 1000 with 10ml pump head
- the Degasser Smartline Manager 5000
- a Smartline Autosampler 3900
- a Jetstream column oven
- a Smartline UV-Detector 2600
- a Smartline RI-Detector 2300.
![Analytische HPLC-Anlage 1 Analytische HPLC-Anlage 1](/storages/ccb/_processed_/9/7/csm_Anlage-1_50224724f1.jpg)
The second system also consists of:
- a Smartline pump 1000 with 10ml pump head
- the Degasser Smartline Manager 5000
- a Smartline Autosampler 3900
- a Smartline UV-Detector 2600
- a Smartline RI-Detector 2300.
![Analytische HPLC-Anlage 2 Analytische HPLC-Anlage 2](/storages/ccb/_processed_/e/1/csm_Anlage-2_bc04cfa5e8.jpg)
Preparative HPLC system
The system consists of:
- a Smartline UV-Detector 2600
- a preparative Pump 1800 with 100ml pump head
- a Feed-pump Smartline Valve Drive with Smartline Pump 100 with 10 ml pump head.
![Präparative HPLC-Anlage Präparative HPLC-Anlage](/storages/ccb/_processed_/3/c/csm_Anlage-3_621559316b.jpg)
Semi-preparative HPLC systems
The first system consists of:
- a Smartline UV Detector 2600
- two high pressure gradient pumps Smartline Pump 1000 with 50ml pump head
- the Degasser Smartline Manager 5000
- a Feed-Pump Smartline Valve Drive with Smartline Pump 100.
![Semipräparative HPLC-Anlage 1 Semipräparative HPLC-Anlage 1](/storages/ccb/_processed_/a/5/csm_Anlage-4_577cdb02cb.jpg)
The second system is identical in construction. Furthermore, the peaks can be collected with the fraction collector Foxy R1.
![Semipräparative HPLC-Anlage 2 Semipräparative HPLC-Anlage 2](/storages/ccb/_processed_/d/d/csm_Anlage-5_31ee720508.jpg)
Software
The analytical systems and the semi-preparative system with fraction collector Foxy R1 are operated with the software Clarity Chrom Version 7.2.0.73, the semi-preparative system without fraction collector and preparative system are operated with the software Clarity Preparative Version 7.2.0.73.
The LC-MS system is operated with the software Empower 3 Version Fr2.
HPLC-MS
The HPLC-MS system consists of:
- a Waters Alliance e2695 Separation Module
- a 2998 PDA Detector
- the ACQUITY QDa Detector, it is a single quadrupole mass spectrometer with ESI source
The mass range of the system is between m/z: 30 and 1250. - TLC-MS interface of the brand Camag.
![HPLC/MS HPLS/MS](/storages/ccb/_processed_/b/c/csm_Anlage-6_fb3c2d0939.jpg)
Auto sampler
![Autosampler Autosampler](/storages/ccb/_processed_/9/d/csm_Autosampler_2e6a58d205.jpg)