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HPLC/Mass Spectrometry

Lab management: Stephanie Schulz

The HPLC/MS department is part of the organic chemistry, but samples are accepted and processed by the entire department.

The equipment includes one preparative, two analytical and two semi-preparative HPLC systems from Knauer. Furthermore, a Waters HPLC system with coupled mass spectrometer and TLC-MS interface is available.

Analytical HPLC systems

The first system consists of:

  • a Smartline Pump 1000 with 10ml pump head
  • the Degasser Smartline Manager 5000
  • a Smartline Autosampler 3900
  • a Jetstream column oven
  • a Smartline UV-Detector 2600
  • a Smartline RI-Detector 2300.
Analytische HPLC-Anlage 1 © Ralf Maserski​/​TU-Dortmund

The second system also consists of:

  • a Smartline pump 1000 with 10ml pump head
  • the Degasser Smartline Manager 5000
  • a Smartline Autosampler 3900
  • a Smartline UV-Detector 2600
  • a Smartline RI-Detector 2300.
Analytische HPLC-Anlage 2 © Ralf Maserski​/​TU-Dortmund

Preparative HPLC system

The system consists of:

  • a Smartline UV-Detector 2600
  • a preparative Pump 1800 with 100ml pump head
  • a Feed-pump Smartline Valve Drive with Smartline Pump 100 with 10 ml pump head.
Präparative HPLC-Anlage © Ralf Maserski​/​TU-Dortmund

Semi-preparative HPLC systems

The first system consists of:

  • a Smartline UV Detector 2600
  • two high pressure gradient pumps Smartline Pump 1000 with 50ml pump head
  • the Degasser Smartline Manager 5000
  • a Feed-Pump Smartline Valve Drive with Smartline Pump 100.
Semipräparative HPLC-Anlage 1 © Ralf Maserski​/​TU-Dortmund

The second system is identical in construction. Furthermore, the peaks can be collected with the fraction collector Foxy R1.

Semipräparative HPLC-Anlage 2 © Ralf Maserski​/​TU-Dortmund

Software

Software

The analytical systems and the semi-preparative system with fraction collector Foxy R1 are operated with the software Clarity Chrom Version 7.2.0.73, the semi-preparative system without fraction collector and preparative system are operated with the software Clarity Preparative Version 7.2.0.73.

The LC-MS system is operated with the software Empower 3 Version Fr2.


HPLC-MS

The HPLC-MS system consists of:

  • a Waters Alliance e2695 Separation Module
  • a  2998 PDA Detector
  • the ACQUITY QDa Detector,  it is a single quadrupole mass spectrometer with ESI source
    The mass range of the system is between m/z: 30 and 1250.
  • TLC-MS interface of the brand Camag.
HPLS/MS © Ralf Maserski​/​TU-Dortmund

Auto sampler

Autosampler © Ralf Maserski​/​TU-Dortmund