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HPLC/Mass Spectrometry

Lab management: Stephanie Schulz

The HPLC/MS de­part­ment is part of the organic chem­is­try, but samples are accepted and processed by the entire de­part­ment.

The equipment includes one preparative, two analytical and two semi-preparative HPLC systems from Knauer. Furthermore, a Waters HPLC system with coupled mass spectrometer and TLC-MS interface is available.

Analytical HPLC systems

The first system consists of:

  • a Smartline Pump 1000 with 10ml pump head
  • the Degasser Smartline Manager 5000
  • a Smartline Autosampler 3900
  • a Jetstream column oven
  • a Smartline UV-Detector 2600
  • a Smartline RI-Detector 2300.
Analytische HPLC-Anlage 1 © Ralf Maserski​/​TU-Dort­mund

The second system also consists of:

  • a Smartline pump 1000 with 10ml pump head
  • the Degasser Smartline Manager 5000
  • a Smartline Autosampler 3900
  • a Smartline UV-Detector 2600
  • a Smartline RI-Detector 2300.
Analytische HPLC-Anlage 2 © Ralf Maserski​/​TU-Dort­mund

Preparative HPLC system

The system consists of:

  • a Smartline UV-Detector 2600
  • a preparative Pump 1800 with 100ml pump head
  • a Feed-pump Smartline Valve Drive with Smartline Pump 100 with 10 ml pump head.
Präparative HPLC-Anlage © Ralf Maserski​/​TU-Dort­mund

Semi-preparative HPLC systems

The first system consists of:

  • a Smartline UV Detector 2600
  • two high pressure gradient pumps Smartline Pump 1000 with 50ml pump head
  • the Degasser Smartline Manager 5000
  • a Feed-Pump Smartline Valve Drive with Smartline Pump 100.
Semipräparative HPLC-Anlage 1 © Ralf Maserski​/​TU-Dort­mund

The second system is identical in construction. Furthermore, the peaks can be collected with the fraction collector Foxy R1.

Semipräparative HPLC-Anlage 2 © Ralf Maserski​/​TU-Dort­mund

Soft­ware

The analytical systems and the semi-preparative system with fraction collector Foxy R1 are operated with the software Clarity Chrom Version 7.2.0.73, the semi-preparative system without fraction collector and preparative system are operated with the software Clarity Preparative Version 7.2.0.73.

The LC-MS system is operated with the software Empower 3 Version Fr2.


HPLC-MS

The HPLC-MS system consists of:

  • a Waters Alliance e2695 Separation Module
  • a  2998 PDA Detector
  • the ACQUITY QDa Detector,  it is a single quadrupole mass spectrometer with ESI source
    The mass range of the system is between m/z: 30 and 1250.
  • TLC-MS interface of the brand Camag.
HPLS/MS © Ralf Maserski​/​TU-Dort­mund

Auto sampler

Autosampler © Ralf Maserski​/​TU-Dort­mund

Location & approach

The campus of TU Dort­mund Uni­ver­sity is located close to interstate junction Dort­mund West, where the Sauerlandlinie A 45 (Frankfurt-Dort­mund) crosses the Ruhrschnellweg B 1 / A 40. The best interstate exit to take from A 45 is "Dort­mund-Eichlinghofen" (closer to Cam­pus Süd), and from B 1 / A 40 "Dort­mund-Dorstfeld" (closer to Cam­pus Nord). Signs for the uni­ver­si­ty are located at both exits. Also, there is a new exit before you pass over the B 1-bridge leading into Dort­mund.

To get from Cam­pus Nord to Cam­pus Süd by car, there is the connection via Vo­gel­pothsweg/Baroper Straße. We recommend you leave your car on one of the parking lots at Cam­pus Nord and use the H-Bahn (suspended monorail system), which conveniently connects the two campuses.

TU Dort­mund Uni­ver­sity has its own train station ("Dort­mund Uni­ver­si­tät"). From there, suburban trains (S-Bahn) leave for Dort­mund main station ("Dort­mund Hauptbahnhof") and Düsseldorf main station via the "Düsseldorf Airport Train Station" (take S-Bahn number 1, which leaves every 20 or 30 minutes). The uni­ver­si­ty is easily reached from Bochum, Essen, Mülheim an der Ruhr and Duis­burg.

You can also take the bus or subway train from Dort­mund city to the uni­ver­si­ty: From Dort­mund main station, you can take any train bound for the Station "Stadtgarten", usually lines U41, U45, U 47 and U49. At "Stadtgarten" you switch trains and get on line U42 towards "Hombruch". Look out for the Station "An der Palmweide". From the bus stop just across the road, busses bound for TU Dort­mund Uni­ver­sity leave every ten minutes (445, 447 and 462). Another option is to take the subway routes U41, U45, U47 and U49 from Dort­mund main station to the stop "Dort­mund Kampstraße". From there, take U43 or U44 to the stop "Dort­mund Wittener Straße". Switch to bus line 447 and get off at "Dort­mund Uni­ver­si­tät S".

The H-Bahn is one of the hallmarks of TU Dort­mund Uni­ver­sity. There are two stations on Cam­pus Nord. One ("Dort­mund Uni­ver­si­tät S") is directly located at the suburban train stop, which connects the uni­ver­si­ty directly with the city of Dort­mund and the rest of the Ruhr Area. Also from this station, there are connections to the "Technologiepark" and (via Cam­pus Süd) Eichlinghofen. The other station is located at the dining hall at Cam­pus Nord and offers a direct connection to Cam­pus Süd every five minutes.

The AirportExpress is a fast and convenient means of transport from Dort­mund Airport (DTM) to Dort­mund Central Station, taking you there in little more than 20 minutes. From Dort­mund Central Station, you can continue to the uni­ver­si­ty campus by interurban railway (S-Bahn). A larger range of in­ter­na­tio­nal flight connections is offered at Düsseldorf Airport (DUS), which is about 60 kilometres away and can be directly reached by S-Bahn from the uni­ver­si­ty station.

The facilities of TU Dort­mund Uni­ver­sity are spread over two campuses, the larger Cam­pus North and the smaller Cam­pus South. Additionally, some areas of the uni­ver­si­ty are located in the adjacent "Technologiepark".

Site Map of TU Dort­mund Uni­ver­sity (Second Page in English).