de Capitani, J., Mutschler, H.
The Long Road to a Synthetic Self-Replicating Central Dogma.
Biochemistry in press (2023).
Salditt, A., Karr, L., Salibi, E., Le Vay, K., Braun, D.*, Mutschler, H.*
Ribozyme-mediated RNA synthesis and replication in a model Hadean microenvironment.
Nat Commun 14, 1495 (2023).
Salibi, E.#, Peter, B. #, Schwille, P.*, Mutschler, H.*
Periodic temperature changes drive the proliferation of self-replicating RNAs in vesicle populations.
Nat Commun 14, 1222 (2023).
Hürtgen, D., Mascarenhas, J. #, Vibhute, M. A. #, Weise, L. I. Mayr, V. S., Sourjik, V.*, Mutschler, H.*
Engineering a Synthetic RNA Segregation System.
ChemSystemsChem, e202200028 (2023).
Vibhute, M. A. *, Mutschler, H.*
A Primer on Building Life‐Like Systems.
ChemSystemsChem 5, e202200033 (2023).
Le Vay K., Salibi E., Ghosh B., Tang TY-D.*, Mutschler H.*
Ribozyme-phenotype coupling in peptide-based coacervate protocells
Salditt A., Karr L., Salibi E., Braun D.*, Mutschler H.*
Complete RNA replication cycles in a Hadean microcompartment,
19 September 2022, PREPRINT (Version 1) available at Research Square
Salibi E., Peter B., Schwille P.*, Mutschler H.*
Exchange, catalysis and amplification of encapsulated RNA driven by periodic temperature changes,
12 September 2022, PREPRINT (Version 1) available at Research Square
Wagner, A., Weise, L.I. & Mutschler, H.
In vitro characterisation of the MS2 RNA polymerase complex reveals host factors that modulate emesviral replicase activity.
Communications Biology, 2022, 5, 264
Szokoli D., Le Vay K.K., Mutschler, H.
PCR Methods for the Generation of Catalytic ssDNA.
Methods in Molecular Biology, 2022, 2439, 27-44
Le Vay K.K. & Mutschler, H.
Generation of RNA with 2′, 3′-Cyclic Phosphates by Deoxyribozyme Cleavage in Frozen Solutions
Methods in Molecular Biology, 2022, 2439, 301-309
Iglesias-Artola, J.M., Drobot, B., Kar, M., Tang, T-YD., Mutschler, H. Kreysing, M.
Charge-density reduction promotes ribozyme activity in RNA–peptide coacervates via RNA fluidization and magnesium partitioning.
Nature Chemistry, 2022, in press
Le Vay K*#, Song EY#, Ghosh B, Tang T-YD*, Mutschler H*.
Enhanced ribozyme-catalyzed recombination and oligonucleotide assembly in peptide-RNA condensates.
Angewandte Chemie International Edition, 2021, 60, 26096-26104
*corresponding, #contributed equally
Matreux T#, Le Vay K#, Schmid A, Aikkila P, Belohlavek L, Çalışkanoğlu AZ, Salibi E, Kühnlein A, Springsklee C, Scheu B, Dingwell DB, Braun D, Mutschler H*, Mast CB*.
Heat flows in rock cracks naturally optimize salt compositions for ribozymes.
Nature Chemistry (2021).
#contributed equally, *corresponding authors
Wagner A, Weise L, Mutschler H.
In vitro characterisation of the MS2 RNA polymerase complex reveals novel host factors that modulate leviviral replicase activity,
12 April 2021, PREPRINT (Version 1) available at Research Square
Libicher K, Mutschler H.
Probing self-regeneration of essential protein factors required for in vitro translation activity by serial transfer (2020),
Chemical Communications, 2020, 56, 15426-15429
Song EY, Jiménez EI, Lin H, Le Vay, K, Krishnamurthy R*, Mutschler H*.
Prebiotically plausible RNA activation compatible with ribozyme‐catalyzed ligation (2020),
Angewandte Chemie International Edition, 2020, 60, 2952-2957
Donau C, Späth F, Sosson M, Kriebisch B, Schnitter F, Tena-Solsona M,Kang HS, Salibi E, Sattler M, Mutschler H, Job Boekhoven J.
Active coacervate droplets as a model for membraneless organelles and protocells (2020),
Nature Communications, 11, 5167
Le Vay K, Mutschler H,
How DNA and RNA subunits might have formed to make the first genetic alphabet (2020),
Nature, 582, 33-34
Libicher K, Hornberger R, Heymann M, Kriebisch B, Mutschler H.
In vitro self-replication and multicistronic expression of large synthetic genomes (2020),
Nature Communications, 11, 904
Le Vay K*, Salibi E#, Song Y#, Mutschler H*.
Nucleic acid catalysis under potential prebiotic conditions (2019),
Chemistry—An Asian Journal, https://doi.org/10.1002/asia.201901205
*corresponding authors, #contributed equally
Mutschler H*, Robinson T*, Tang TYD*, Wegner S*.
Special Issue on Bottom‐Up Synthetic Biology (2019),
ChemBioChem, 20, 2533-2534
Weise LI, Heymann M, Mayr V, Mutschler H.
Cell-free expression of RNA encoded genes using MS2 replicase (2019),
Nucleic Acids Research, gkz817
Le Vay K and Mutschler H.
The difficult case of an RNA-only origin of life (2019)
Emerging Topics in Life Sciences, ETLS20190024
Morasch M, Liu J, Dirscherl C, Ianeselli A, Kühnlein A, Le Vay K, Schwintek P, Islam S, Corpinot M, Scheu B, Dingwell D, Schwille P, Mutschler H, Powner M, Mast C, and Braun D.
Heated gas bubbles enrich, crystallize, dry, phosphorylate, and encapsulate prebiotic molecules (2019)
Nature Chemistry, 11, 779-788
Le Vay K, Weise LI, Libicher K, Mascarenhas J, Mutschler H.
Templated Self‐Replication in Biomimetic Systems (2019)
Advanced BioSystems, 3, 1800313
Mutschler H#*, Taylor AI*, Porebski BT, Lightowlers A, Houlihan G, Abramov M, Herdewijn P, Holliger P#.
Random-sequence genetic oligomer pools display an innate potential for ligation and recombination (2018)
#corresponding authors, *joint first-authors
Drobot B, Iglesias-Artola JM*, Le Vay K*, Mayr V, Kar M, Kreysing M#, Mutschler H#, Tang TYD#.
Compartmentalized RNA catalysis in membrane - free coacervate protocells (2018)
Nature Communications, 9, 3643
#corresponding authors, *contributed equally
Weise LI, Libicher K, Mutschler H.
Copy, paste, repeat – über die Synthese von Minimalzellen (2018)
BIOspektrum, 4, 365-367.
Schwille P, Spatz J, Landfester K, Bodenschatz E, Herminghaus S, Sourjik V, Erb T, Bastiaens P, Lipowsky R, Hyman A, Dabrock P, Baret JC, Vidakovic-Koch T, Bieling P, Dimova R, Mutschler H, Robinson T, Tang D, Wegner S, Sundmacher K.
MaxSynBio ‐ Avenues towards creating cells from the bottom up (2018)
Angewandte Chemie International Edition, 130, 13566-13577
Litschel T, Ganzinger KA, Movinkel T, Heymann M, Robinson T, Mutschler H*, Schwille P*.
Freeze-thaw cycles induce content exchange between cell-sized lipid vesicles (2018)
New Journal of Physics, 20, 055008
Cozens C*, Mutschler H*, Nelson GM, Houlihan G, Taylor AI, Holliger P.
Enzymatic Synthesis of Nucleic Acids with Defined Regioisomeric 2′-5′ Linkages (2015)
Angewandte Chemie International Edition, 54, 15570–15573
Mutschler H, Wochner A, Holliger P.
Freeze-thaw cycles as drivers of complex ribozyme assembly. (2015)
Nature Chemistry, 7, 502–508
Mutschler H, Holliger P.
Non-canonical 3'-5' extension of RNA with prebiotically plausible ribonucleoside 2', 3'-cyclic phosphates. (2014)
Journal of the American Chemical Society, 136, 5193–5196.
Mutschler H, Meinhart A.
ε/ζ systems: their role in resistance, virulence, and their potential for antibiotic development. (2011)
Journal of Molecular Medicine, 89, 1183-1194.
Mutschler H, Gebhardt M, Shoeman RL, Meinhart A.
A novel mechanism of programmed cell death in bacteria by toxin-antitoxin systems corrupts peptidoglycan synthesis. (2011)
PLoS Biology, 9, e1001033.
Lunde BM, Reichow SL, Kim M, Suh H, Leeper TC, Yang F, Mutschler H, Buratowski S, Meinhart A, Varani G.
Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain. (2010)
Nature Structural & Molecular Biology, 17, 1195-1201.
Mutschler H, Reinstein J, Meinhart A.
Assembly dynamics and stability of the pneumococcal epsilon zeta antitoxin toxin (PezAT) system from Streptococcus pneumoniae. (2010)
Journal of Biological Chemistry, 285, 21797-806.
Kress W, Mutschler H, Weber-Ban E.
Both ATPase domains of ClpA are critical for processing of stable protein structures. (2009)
Journal of Biological Chemistry, 284, 31441-52.
Vasiljeva L, Kim M*, Mutschler H*, Buratowski S, Meinhart A.
The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. (2008)
Nature Structural & Molecular Biology, 15, 795-804.
Kress W, Mutschler H, Weber-Ban E.
Assembly pathway of an AAA+ protein: tracking ClpA and ClpAP complex formation in real time. (2007)
Biochemistry, 46, 6183-93.
Use of a new class of nucleotide sugar as antibiotic or cytostatic agent, Mutschler H, Meinhart A. WO Patent 2,012,038,097 (2012)
Mutschler H, Meinhart A.
Type II Toxin-Antitoxin Loci: The Epsilon/Zeta Family
in: Prokaryotic Toxin-Antitoxins, 205-223. Springer Berlin Heidelberg (2013)
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Location & approach
The campus of TU Dortmund University is located close to interstate junction Dortmund West, where the Sauerlandlinie A 45 (Frankfurt-Dortmund) crosses the Ruhrschnellweg B 1 / A 40. The best interstate exit to take from A 45 is "Dortmund-Eichlinghofen" (closer to Campus Süd), and from B 1 / A 40 "Dortmund-Dorstfeld" (closer to Campus Nord). Signs for the university are located at both exits. Also, there is a new exit before you pass over the B 1-bridge leading into Dortmund.
To get from Campus Nord to Campus Süd by car, there is the connection via Vogelpothsweg/Baroper Straße. We recommend you leave your car on one of the parking lots at Campus Nord and use the H-Bahn (suspended monorail system), which conveniently connects the two campuses.
TU Dortmund University has its own train station ("Dortmund Universität"). From there, suburban trains (S-Bahn) leave for Dortmund main station ("Dortmund Hauptbahnhof") and Düsseldorf main station via the "Düsseldorf Airport Train Station" (take S-Bahn number 1, which leaves every 20 or 30 minutes). The university is easily reached from Bochum, Essen, Mülheim an der Ruhr and Duisburg.
You can also take the bus or subway train from Dortmund city to the university: From Dortmund main station, you can take any train bound for the Station "Stadtgarten", usually lines U41, U45, U 47 and U49. At "Stadtgarten" you switch trains and get on line U42 towards "Hombruch". Look out for the Station "An der Palmweide". From the bus stop just across the road, busses bound for TU Dortmund University leave every ten minutes (445, 447 and 462). Another option is to take the subway routes U41, U45, U47 and U49 from Dortmund main station to the stop "Dortmund Kampstraße". From there, take U43 or U44 to the stop "Dortmund Wittener Straße". Switch to bus line 447 and get off at "Dortmund Universität S".
The H-Bahn is one of the hallmarks of TU Dortmund University. There are two stations on Campus Nord. One ("Dortmund Universität S") is directly located at the suburban train stop, which connects the university directly with the city of Dortmund and the rest of the Ruhr Area. Also from this station, there are connections to the "Technologiepark" and (via Campus Süd) Eichlinghofen. The other station is located at the dining hall at Campus Nord and offers a direct connection to Campus Süd every five minutes.
The AirportExpress is a fast and convenient means of transport from Dortmund Airport (DTM) to Dortmund Central Station, taking you there in little more than 20 minutes. From Dortmund Central Station, you can continue to the university campus by interurban railway (S-Bahn). A larger range of international flight connections is offered at Düsseldorf Airport (DUS), which is about 60 kilometres away and can be directly reached by S-Bahn from the university station.
The facilities of TU Dortmund University are spread over two campuses, the larger Campus North and the smaller Campus South. Additionally, some areas of the university are located in the adjacent "Technologiepark".
Site Map of TU Dortmund University (Second Page in English).