*: Corresponding Author, §: Equally Contributed
81. A. Witte, A. Munoz-Lopez, M. Metz, M. R. Schweiger, P. Janning and D. Summerer*
Encoded, Click-Reactive DNA-Binding Domains for Programmable Capture of Specific Chromatin Segments
Chem. Sci., 2020, accepted.
80. A. Munoz-Lopez, A. Jung, B. Buchmuller, J. Wolffgramm, S. Maurer, A. Witte and D. Summerer*
Engineered TALE Repeats for Enhanced Imaging-based Analysis of Cellular 5-Methylcytosine
ChemBioChem, 2020, accepted.
79. S. Palei, B. Buchmuller, J. Wolffgramm, A. Munoz-Lopez, S. Jung, P. Czodrowski, D. Summerer*
Light-Activatable TET-Dioxygenases Reveal Dynamics of 5-Methylcytosine Oxidation and Transcriptome Reorganization
J. Am. Chem. Soc., 2020, doi.org/10.1021/jacs.0c01193
78. B. Buchmuller, B. Kosel, D. Summerer*
Complete Profiling of Methyl-CpG-Binding Domains for Combinations of Cytosine Modifications at CpG Dinucleotides Reveals Differential Read-out in Normal and Rett-Associated States
Sci. Rep., 2020, 10, 4053.
77. A. Munoz-Lopez, B. Buchmuller, J. Wolffgramm, A. Jung, M. Hussong, J. Kanne, M. R. Schweiger* and D. Summerer*
Designer Receptors for Nucleotide Resolution Analysis of Genomic 5-Methylcytosine by Cellular Imaging
Angew. Chem. Int. Ed., 2020, doi.org/10.1002/anie.202001935
76. B. Buchmuller, D. Summerer
Method for determining 5-methylcytosine configurations in DNA
Patent Application, 2020, 19 220 082.2. filed by the TU Dortmund.
75. P. Widder, J. Schuck, D. Summerer* and M. Drescher*
Combining site-directed spin labeling in vivo and in-cell EPR Distance Determination
Phys. Chem. Chem. Phys., 2020, 22, 4875-9.
74. B. Buchmuller, A. Munoz-Lopez, M. Giess and D. Summerer*
Design and application of DNA modification-specific transcription-activator-like effectors
Meth. Mol. Biol., 2020, in press.
73. T. Braun, P. Widder, U. Osswald, L. Groß, L. Williams, M. J. Schmidt, I. Helmle, D. Summerer* and Malte Drescher*
Isoindoline-Based Nitroxides as Bioresistant Spin Labels for Protein Labeling via Cysteines and Alkyne bearing Noncanonical Amino Acids
ChemBioChem, 2019, doi: 10.1002/cbic.201900537.
72. M. Giess, A. Munoz-Lopez, B. Buchmuller, G. Kubik and D. Summerer*
Programmable Protein-DNA Crosslinking for the Direct Capture and Quantification of 5-Formylcytosine
J. Am. Chem. Soc., 2019, 141, 9453-7.
71. P. Widder, F. Berner, D. Summerer* and M. Drescher*
Double Nitroxide Labeling by Copper-Catalyzed Azide-Alkyne Cycloadditions with Noncanonical Amino Acids for EPR Spectroscopy
ACS Chem. Biol., 2019,14(5), 839-44.
70. A. Kugele, T. Braun, P. Widder, L. Williams, M. J. Schmidt, D. Summerer*, M. Drescher*
Site-Directed spin labelling of proteins by Suzuki-Miyaura coupling via a genetically encoded aryliodide amino acid
Chem. Commun., 2019, 55(13), 1923-1926.
69. T. Braun, M. Drescher* and D. Summerer*
Expanding the genetic code for site-directed spin-labeling
Int. J. Mol. Sci. ., 2019, 9, 20(2), E373. (Special Issue on “Expanding and Reprogramming the Genetic Code”, edited by K. Sakamoto).
68. S. Maurer, B. Buchmuller, C. Ehrt, J. Jasper, O. Koch and D. Summerer*
Overcoming conservation in TALE-DNA interactions: A minimal repeat scaffold enables selective rerognition of an oxidized 5-methylcytosine
Chem. Sci., 2018, 9, 7247-52.
67. M. Gieß, A. Witte, J. Jasper, O. Koch and D. Summerer*
Complete programmable decoding of oxidized 5-methylcytosine nucleobases in DNA by chemoselective blockage of universal transcription-activator-like effector repeats
J. Am. Chem. Soc., 2018, 140, 5904-8.
66. H. Neumann*, P. Neumann-Staubitz, A. Witte and D. Summerer*
Epigenetic chromatin modification by amber suppression technology
Curr. Opin. Chem. Biol., 2018, 45, 1-9.
65. M. J. Schmidt, and D. Summerer*
Directed evolution of orthogonal pyrrolysyl-tRNA synthetases in Escherichia coli for the genetic encoding of noncanonical amino acids
Meth. Mol. Biol., 2018, 1728, 97-111.
64. A. Munoz-Lopez and D. Summerer*
Recognition of oxidized 5-methylcytosine derivatives in DNA by natural and engineered protein scaffolds
Chem. Rec., 2018, 18(1), 105-16.
63. P. Rathi, S. Maurer and D. Summerer*
Selective recognition of N4-methylcytosine in DNA by engineered transcription-activator-like effectors
Philos. Trans. R. Soc. Lond. B Biol. Sci., 2018, 373, 1748. (Special Issue on “‘Frontiers in Epigenetic Chemical Biology”).
62. P. Rathi, A. Witte and D. Summerer*
Engineering DNA backbone interactions results in TALE scaffolds with enhanced 5-methylcytosine selectivity
Sci. Rep., 2017, 7, 15067.
61. S. Flade, J. Jasper, M. Giess, M. Juhasz, A. Dankers, G. Kubik, O. Koch*, E. Weinhold* and D. Summerer*
The N6-position of adenine is a blind spot for TAL-effectors that enables effective binding of methylated and fluorophore-labeled DNA
ACS Chem. Biol., 2017, 12 (7), 1719-25.
60. S. Maurer, M. Giess, O. Koch and D. Summerer*
Interrogating key positions of size-reduced TALE-repeats reveals a programmable sensor of 5-carboxylcytosine
ACS Chem. Biol., 2016,11 (12), 3294-9.
59. Y. J. Lee, M. J. Schmidt, J. M. Tharp, A. Weber, J. Gao, M. L. Waters, D. Summerer* and W. R. Liu*
Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader
Chem. Commun., 2016, 52 (85), 12606-9.
58. P. Rathi, S. Maurer, G. Kubik and D. Summerer*
Isolation of human genomic DNA sequences with expanded nucleobase selectivity
J. Am. Chem. Soc., 2016,138, 9910-18.
57. P. Roser, M. J. Schmidt, M. Drescher* and D. Summerer*
Site-directed spin labeling of proteins for distance measurements in vitro and in cells
Org. Biomol. Chem., 2016,14, 5468-76.
56.G. Kubik and D. Summerer*
TALEored epigenetics: a DNA-binding scaffold for programmable epigenome editing and analysis
ChemBioChem., 2016,17, 975-80.
55. D. Summerer*, M. Giess, S. Maurer, G. Kubik
Transcription activator-like effector (TALE)-based decoding of cytosine nucleobases by selective modification response
Patent application., 2016, filed by the University of Konstanz.
54. M. J. Schmidt, A. Fedoseev, D. Bücker, J. Borbas, C. Peter, M. Drescher* and D. Summerer*
EPR Distance measurements in native proteins with genetically encoded spin labels
ACS Chem. Biol., 2015, 18, 2764-71.
53. D. Summerer*
N6-Methyladenine: a potential epigenetic mark in eukaryotic genomes
Angew. Chem. Int. Ed., 2015, 54, 10714-6.
52. M. J. Schmidt, A. Fedoseev, D. Summerer* and M. Drescher*
Genetically encoded spin labels for in vitro and in-cell EPR studies of native proteins
Methods Enzymol., 2015, 563, 483-502.
51. G. Kubik and D. Summerer*
Deciphering epigenetic cytosine modifications by direct molecular recognition
ACS Chem. Biol., 2015, 10, 1580-9.
50. G. Kubik, S. Batke and D. Summerer*
Programmable sensors of 5-hydroxymethylcytosine
J. Am. Chem. Soc., 2015, 137, 2-5.
49. G. Kubik and D. Summerer*
Achieving single nucleotide resolution of 5-methylcytosine detection with TALEs
ChemBioChem., 2015, 16, 228-31.
48. B. Klauser, C. Rehm, D. Summerer and J. S. Hartig
Engineering of ribozyme-based aminoglycoside switches of gene expression by in vivo genetic selection in Saccharomycesc erevisiae
Methods Enzymol., 2015, 550, 301-20.
47. D. Summerer, M. J. Schmidt, M. Drescher
Genetically encoded spin label
Patent application, 2015, PCT/EP2015/050568, filed by the University of Konstanz
46. M. Pott, M. J. Schmidt and D. Summerer*
Evolved sequence contexts for highly efficient amber suppression with noncanonical amino acids
ACS Chem. Biol., 2014, 9, 2815-22.
45. G. Kubik, M. J. Schmidt, J. E. Penner and D. Summerer*
Programmable and highly resolved in vitro detection of genomic 5-methylcytosine by TALEs
Angew. Chem. Int. Ed., 2014, 53, 6002-6.
44. M. J. Schmidt, A. Weber, M. Pott, W. Welte and D. Summerer*
Structural basis of furan-amino acid recognition by a polyspecific aminoacyl-tRNA-synthetase and its genetic encoding in human cells
ChemBioChem, 2014, 15, 1755-60.
43. M. J. Schmidt and D. Summerer*
Genetic code expansion as a tool to study regulatory processes of transcription
Front. Chem., 2014, 2 (7), 1-11.
42. M. J. Schmidt, J. Borbas, M. Drescher* and D. Summerer*
A genetically encoded spin label for electron paramagnetic resonance distance measurements
J. Am. Chem. Soc., 2014, 136, 1238-41.
Three times highlighted by Faculty of 1000 (including rating: “exceptional”)
41. D. Summerer, M. J. Schmidt
Intercalating amino acids
Patent application, 2014, PCT/EP2014/065601, filed by the University of Konstanz
40. D. Summerer, G. Kubik, M. J. Schmidt
Direct, programmable detection of epigenetic cytosine modifications using TAL effectors
Patent application, 2014, PCT/EP2014/001753, filed by the University of Konstanz
39. M. J. Schmidt, D. Summerer*
Red-light-controlled protein-RNA crosslinking with a genetically encoded furan
Angew. Chem. Int. Ed., 2013, 52, 4690-93.
38. M. J. Schmidt, D. Summerer*
A need for speed: genetic encoding of rapid cycloaddition chemistries for protein labelling in living cells
ChemBioChem, 2012, 13, 1553-57.
37. D. Summerer*
High-throughput DNA sequencing beyond the four-letter code: epigenetic modifications revealed by single molecule bypass kinetics
ChemBioChem, 2010, 11, 2499-501
36. D. Summerer*, D. Hevroni, A. Jain, O. Scheck, J. Parker, A. Caruso, P.F. Stähler, C.F. Stähler and M. Beier
A flexible and fully integrated system for amplification, detection and genotyping of genomic DNA targets based on microfluidic oligonucleotide arrays
N. Biotechnology, 2010, 27, 149-55
35. M. Beier, P. F. Stähler, C.F. Stähler, J. Leonhard, D. Summerer, S. Bau
Process for the investigation of nucleic acid populations
Patent application, 2010, DE102008061772A1, filed by febit holding gmbh
34. D. Summerer*, N. Schracke, H. Wu, Y. Cheng, S. Bau, C.F. Stähler, P.F. Stähler and M. Beier
Targeted enrichment of cancer-related gene exons with a flexible and fully automated microarray platform
Genomics, 2010, 95, 241-6
33. C.F. Stähler, P.F. Stähler, M. Beier, D. Summerer
Integrated amplification, processing and analysis of biomolecules in a microfluidic reaction medium
Patent application, 2010, WO2010043418, filed by febit holding gmbh
32. D. Summerer*
Enabling technologies of genomic-scale sequence enrichment for high throughput sequencing
Genomics, 2009, 94, 363-8
31. N. Schracke, M. Kränzle, P. F. Stähler, D. Summerer and M. Beier
Specific sequence selection and next generation resequencing of 68 E. coli genes using HybSelect
N. Biotechnology, 2009, 26, 229-33
30. D. Summerer*, H. Wu, B. Haase, Y. Cheng, N. Schracke, C.F. Stähler, M.S. Chee, P.F. Stähler and M. Beier
Microarray-based multicycle enrichment of genomic loci for next-generation-sequencing
Genome Res., 2009, 19, 1616-21
Described methodology was highlighted in the Faculty of 1000s magazine The Scientist: “The ten most exciting tools to hit the life sciences in 2009″
29. S. Bau, N. Schracke, M. Kränzle, H. Wu, J. D. Hoheisel, P. F. Stähler, M. Beier and D. Summerer*
Targeted next-generation-sequencing by specific capture of multiple genomic loci using microfluidic low-volume DNA arrays
Anal. Bioanal. Chem., 2009, 393, 171-5
28. C.F. Stähler, P.F. Stähler, M. Beier, S. Bau, D. Summerer
Flexible extraction method for the production of sequence-specific molecule libraries
Patent application, 2009, WO2009065620, filed by febit holding gmbh
27. C.F. Stähler, P.F. Stähler, M. Beier, D. Summerer, M. Matzas, S. Vorwerk
Improved molecular biological processing system
Patent application, 2008, WO2008080629, filed by febit holding gmbh
26. D.H. Jones, S.E. Cellitti, X. Hao, Q. Zhang, M. Jahnz, D. Summerer, P.G. Schultz, T. Uno, B.H. Geierstanger
Site-specific labeling of proteins with NMR-active unnatural amino acids
J. Biomol. NMR, 2010, 46, 89-100
25. E.M. Tippmann, W. Liu, D. Summerer, Mack A.V. and P.G. Schultz
A genetically encoded diazirine photocrosslinker in Escherichia Coli
ChemBioChem, 2007, 8, 2210-4
24. E.A. Lemke§, D. Summerer§, B.H. Geierstanger, S.M. Brittain and P.G. Schultz
Control of protein phosphorylation with a genetically encoded photocaged amino acid
Nature Chem. Biol., 2007, 3, 769-72
Faculty of 1000 evaluation: factor 10, exceptional.
Highlighted in Analytical Chemistry, January 1, 2008, “Photocaged amino acids enable scientists to study phosphorylation in vivo“
Highlighted by Arnaud, CH, Chemical & Engineering News, 2007, October 31, “Modified Serine Controls Protein Phosphorylation”
Highlighted by Richards, NGJ, The HFSP Journal, 2008, 2(2), 57-60 “Shining a light on posttranslational modification”
23. D. Summerer, S. Chen, N. Wu, A. Deiters, J.W. Chin and P.G. Schultz
A genetically encoded, fluorescent amino acid
Proc. Nat. Acad. Sci. USA, 2006, 103, 9785-9
Faculty of 1000 evaluation: factor 8, must read
22. Peter G. Schultz, Alexander Deiters, Lital Alfonta, Jonathan R. Chittuluru, Dan Groff, Daniel Summerer, Meng-Lin Tsao, Jiangyun Wang, Ning Wu, Jianming Xie and Huaqiang Zeng
Orthogonal translation components for the in vivo incorporation of unnatural amino acids
Patent application, 2006, WO2006110182, filed by the Scripps Research Institute, licensed to Novartis.
21. M.L. Tsao, D. Summerer, P.G. Schultz
Site-Specific Incorporation of a Distance Probe into Proteins in Escherichia coli
J. Am. Chem. Soc., 2006, 128, 4572-3
20. A. Deiters, T.A. Cropp, D. Summerer, M. Mukherji, P.G. Schultz
Site-specific PEGylation of proteins containing unnatural amino acids
Bioorg. Med. Chem. Lett. 2004, 14, 5743-5
19. D. Summerer*
DNA polymerase profiling
Meth. Mol. Biol., 2008, 225
18. D. Summerer, N.Z. Rudinger, I. Detmer, A. Marx
Enhanced fidelity of mismatch extension by DNA polymerase through directed combinatorial enzyme design
Angew. Chem. Int. Ed., 2005, 44, 4712-5
17. D. Summerer, A. Marx
4´-C-Ethynylthymidine acts as a chain terminator during DNA-synthesis catalyzed by HIV-1 reverse transcriptase
Bioorg. Med. Chem. Lett., 2005, 15, 869-71
16. A. Marx, Nikolas Z. Rudinger, D. Summerer
Mutated DNA Polymerases with increased mispairing discrimination
Patent application, 2005, WO2005074350, filed by the University of Bonn, licensed to Qiagen.
15. A. Marx, D. Summerer
Bigger DNA: New genetic helix with expanded size
Angew. Chem. Int. Ed., 2004, 43, 1625-6
14. M. Strerath, J. Gaster, D. Summerer, A. Marx
Increased single-nucleotide discrimination of PCR by primer probes bearing hydrophobic 4’-C-modifications
ChemBioChem, 2004, 5, 333-9
13. A. Marx, D. Summerer, M. Strerath
Selectivity of DNA replication
Highlights in Bioorganic Chemistry: Methods and Application, Editors: C. Schmuck, H. Wennemers, WILEY-VCH, 2004, 299-308
12. A. Marx, I. Detmer, J. Gaster, D. Summerer
Probing DNA polymerase function with synthetic tools
Synthesis, 2004, 1, 1-14
11. D. Summerer and A. Marx
4′-C-Modified nucleotides as chemical tools for investigation and modulation of DNA polymerase function
Synlett, 2004, 2, 217-24
10. A. Marx, M. Strerath, D. Summerer, A. Pingoud, B. Tews, M. Hahn, P. Friedhoff, J. Wilhelm
Improved method for allele-specific PCR
Patent application, 2003, WO2003072814, filed by Roche Diagnostics gmbh / Hoffmann La Roche
9. B. Tews, J. Wilhelm, D. Summerer, M. Strerath, A. Marx, P. Friedhoff, A. Pingoud, M. Hahn
Application of the C4´-alkylated deoxyribose primer system (CAPS) in allele specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants
Biol. Chem. 2003, 384, 1533-41
8. I. Detmer, D. Summerer, A. Marx
Substrates for investigation of DNA polymerase function: synthesis and properties of 4’-C-alkylated oligonucleotides
Eur. J. Org. Chem. 2003, 10, 1837-46
7. I. Detmer, D. Summerer, A. Marx
DNA minor groove hydration probed with 4’-alkylated thymidines
Chem. Commun. 2002, 20, 2314-5
6. D. Summerer, A. Marx
A molecular beacon for quantitative monitoring of the DNA polymerase reaction in real-time
Angew. Chem. Int. Ed. 2002, 41, 3620-2
5. M. Strerath, D. Summerer, A. Marx
Varied DNA polymerase – sugar interactions in the nucleotide binding pocket
ChemBioChem 2002, 3, 578-80
4. D. Summerer, A. Marx
Differential minor groove interactions between DNA polymerase and sugar backbone of primer and template strands
J. Am. Chem. Soc. 2002, 124, 910-1
3. A. Marx, D. Summerer
Molecular insights into error-prone DNA replication and error-free lesion bypass
ChemBioChem 2002, 3, 405-8
Highlighted by Faculty of 1000
2. D. Summerer, A. Marx
DNA-templated synthesis: more versatile than expected
Angew. Chem. Int. Ed. 2002, 41, 89-90
1. D. Summerer, A. Marx
DNA polymerase selectivity: sugar interactions monitored with high fidelity nucleotides
Angew. Chem. Int. Ed. 2001, 40, 3693-5
Search & People Search
Location & approach
The campus of TU Dortmund University is located close to interstate junction Dortmund West, where the Sauerlandlinie A 45 (Frankfurt-Dortmund) crosses the Ruhrschnellweg B 1 / A 40. The best interstate exit to take from A 45 is "Dortmund-Eichlinghofen" (closer to Campus Süd), and from B 1 / A 40 "Dortmund-Dorstfeld" (closer to Campus Nord). Signs for the university are located at both exits. Also, there is a new exit before you pass over the B 1-bridge leading into Dortmund.
To get from Campus Nord to Campus Süd by car, there is the connection via Vogelpothsweg/Baroper Straße. We recommend you leave your car on one of the parking lots at Campus Nord and use the H-Bahn (suspended monorail system), which conveniently connects the two campuses.
TU Dortmund University has its own train station ("Dortmund Universität"). From there, suburban trains (S-Bahn) leave for Dortmund main station ("Dortmund Hauptbahnhof") and Düsseldorf main station via the "Düsseldorf Airport Train Station" (take S-Bahn number 1, which leaves every 20 or 30 minutes). The university is easily reached from Bochum, Essen, Mülheim an der Ruhr and Duisburg.
You can also take the bus or subway train from Dortmund city to the university: From Dortmund main station, you can take any train bound for the Station "Stadtgarten", usually lines U41, U45, U 47 and U49. At "Stadtgarten" you switch trains and get on line U42 towards "Hombruch". Look out for the Station "An der Palmweide". From the bus stop just across the road, busses bound for TU Dortmund University leave every ten minutes (445, 447 and 462). Another option is to take the subway routes U41, U45, U47 and U49 from Dortmund main station to the stop "Dortmund Kampstraße". From there, take U43 or U44 to the stop "Dortmund Wittener Straße". Switch to bus line 447 and get off at "Dortmund Universität S".
The H-Bahn is one of the hallmarks of TU Dortmund University. There are two stations on Campus Nord. One ("Dortmund Universität S") is directly located at the suburban train stop, which connects the university directly with the city of Dortmund and the rest of the Ruhr Area. Also from this station, there are connections to the "Technologiepark" and (via Campus Süd) Eichlinghofen. The other station is located at the dining hall at Campus Nord and offers a direct connection to Campus Süd every five minutes.
The AirportExpress is a fast and convenient means of transport from Dortmund Airport (DTM) to Dortmund Central Station, taking you there in little more than 20 minutes. From Dortmund Central Station, you can continue to the university campus by interurban railway (S-Bahn). A larger range of international flight connections is offered at Düsseldorf Airport (DUS), which is about 60 kilometres away and can be directly reached by S-Bahn from the university station.
The facilities of TU Dortmund University are spread over two campuses, the larger Campus North and the smaller Campus South. Additionally, some areas of the university are located in the adjacent "Technologiepark".