Small organic compounds that bind to proteins and modulate their function are called bioactive small molecules. They serve as drugs, starting points for drug development, and probes for chemical biology. The identification of bioactive small molecules is one of the most critical steps in a drug discovery program. It is a principal task of medicinal chemists. High throughput screening (HTS), i.e. the screening of large compound libraries at disease-relevant protein targets has proven its value to discover bioactive molecules. These may serve as starting points in drug development programs or as probes in chemical biology. New innovative and broadly applicable concepts to screen libraries of small molecules at disease-relevant proteins are badly needed. The concept to synthesize and pool large combinatorial DNA-encoded small molecule library and to screen the whole encoded library as a pool at immobilized protein targets in a single selection experiment is an innovative approach to bring HTS into academia.
Sequencing of the human genome provided the protein-coding sequences of all genes, including that subset of proteins capable of binding low molecular weight compounds, the so-called “druggable genome”. Typical members of the “druggable genome”, i.e. traditional drug targets, are for example G protein-coupled receptors and enzymes such as kinases. However, the number of proteins associated with diseases is much larger than the druggable genome. Many disease-related proteins, for instance those that take part in protein-protein interactions, are promising, yet difficult targets in drug research. Screening of large DNA-encoded libraries holds promise to identify small molecule ligands for these difficult protein targets.
Encoding of small molecules allows for pooling of small molecule libraries. Putative bioactive small molecules can be identified through an efficient selection assay:
- Incubation of a large pooled encoded small molecule library with an immobilized target protein
- Washing steps to remove the non-binding small molecules
- PCR-amplification of the selected small molecules and Illumina sequencing to identify the protein binders
- Follow-up assays for validation of protein binders and elucidation of mode of action
We are applying a broad range of methods from two very different worlds: chemistry and molecular biology.
- synthesize functionalized small molecule scaffolds by organic preparative chemistry
- couple these scaffolds to DNA
- perform combinatorial chemistry on the DNA-coupled scaffolds
- develop novel DNA-compatible chemical methods to furnish the library
- use methods from molecular biology to encode each chemical step
- develop novel selection assay approaches
in order to
- obtain a large, pooled small molecule library with hundreds of thousands of members
- screen the pooled library at promising drug targets, also from collaboration partners,
to identify novel bioactive small molecules.
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Location & approach
The campus of TU Dortmund University is located close to interstate junction Dortmund West, where the Sauerlandlinie A 45 (Frankfurt-Dortmund) crosses the Ruhrschnellweg B 1 / A 40. The best interstate exit to take from A 45 is "Dortmund-Eichlinghofen" (closer to Campus Süd), and from B 1 / A 40 "Dortmund-Dorstfeld" (closer to Campus Nord). Signs for the university are located at both exits. Also, there is a new exit before you pass over the B 1-bridge leading into Dortmund.
To get from Campus Nord to Campus Süd by car, there is the connection via Vogelpothsweg/Baroper Straße. We recommend you leave your car on one of the parking lots at Campus Nord and use the H-Bahn (suspended monorail system), which conveniently connects the two campuses.
TU Dortmund University has its own train station ("Dortmund Universität"). From there, suburban trains (S-Bahn) leave for Dortmund main station ("Dortmund Hauptbahnhof") and Düsseldorf main station via the "Düsseldorf Airport Train Station" (take S-Bahn number 1, which leaves every 20 or 30 minutes). The university is easily reached from Bochum, Essen, Mülheim an der Ruhr and Duisburg.
You can also take the bus or subway train from Dortmund city to the university: From Dortmund main station, you can take any train bound for the Station "Stadtgarten", usually lines U41, U45, U 47 and U49. At "Stadtgarten" you switch trains and get on line U42 towards "Hombruch". Look out for the Station "An der Palmweide". From the bus stop just across the road, busses bound for TU Dortmund University leave every ten minutes (445, 447 and 462). Another option is to take the subway routes U41, U45, U47 and U49 from Dortmund main station to the stop "Dortmund Kampstraße". From there, take U43 or U44 to the stop "Dortmund Wittener Straße". Switch to bus line 447 and get off at "Dortmund Universität S".
The H-Bahn is one of the hallmarks of TU Dortmund University. There are two stations on Campus Nord. One ("Dortmund Universität S") is directly located at the suburban train stop, which connects the university directly with the city of Dortmund and the rest of the Ruhr Area. Also from this station, there are connections to the "Technologiepark" and (via Campus Süd) Eichlinghofen. The other station is located at the dining hall at Campus Nord and offers a direct connection to Campus Süd every five minutes.
The AirportExpress is a fast and convenient means of transport from Dortmund Airport (DTM) to Dortmund Central Station, taking you there in little more than 20 minutes. From Dortmund Central Station, you can continue to the university campus by interurban railway (S-Bahn). A larger range of international flight connections is offered at Düsseldorf Airport (DUS), which is about 60 kilometres away and can be directly reached by S-Bahn from the university station.
The facilities of TU Dortmund University are spread over two campuses, the larger Campus North and the smaller Campus South. Additionally, some areas of the university are located in the adjacent "Technologiepark".